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Phenol:Chloroform:Isoamyl Alcohol, 25:24:1 100ml
| OmniPur® Phenol:Chloroform:Isoamyl Alcohol, 25:24:1 (TE Buffered Saturated pH 6.7/8.0) - Phenol saturated with TE buffer for DNA isolation. Comes at pH 6.7 for stability with included TE buffer to raise pH to 8.0 for DNA extraction. pH 8.0±0.2: Gently mix contents of phenol: chloroform and buffer bottles. Allow layers to separate before use (2-4 h). pH 6.7±0.2: Use saturated phenol: chloroform as is without addition of buffer bottle. Solution is stable >1 year at 4°C.Phenol saturated with TE buffer for DNA isolation. Comes at pH 6.7 for stability with included TE buffer to raise pH to 8.0 for DNA extraction. Suitable for DNA applications. Molecular biology grade. Specifications: Assay [phenol]: =99.0% pH of Saturated phenol at 25°C: 6.5-6.9 pH of TRIS-EDTA buffer: 7.8-8.2 UV Absorbance at 330 nm: =0.2AU UV Absorbance at 405 nm: =0.1AU UV Absorbance at 510 nm: =0.1AU |
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