| Prev Next |
C18-W HPTLC Plates
| Base material: silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å, specific pore volume 0.75 ml/g, particle size 2 – 10 µm, for preparative plates (1000um thickness of layer) particle size 5 - 17um; pH stability 2 – 10 indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm); UV-absorbing substances apppear as dark-blue to black spots on a light - blue background partial octadecyl (C18) modification, wettable with water, carbon content 14% Order of polarity: Silica > DIOL > NH2 > CN > C2 > C18-50 > C18-W > C18-100 Normal Phase or Reversed Phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water (see table below); the relative polarity of the eluent determines the plarity of the layer Recommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates) As fluorescent indicator, an acid-resistant product is used, which allows application of strongly acidic eluents without undesirable fluorescence quenching. This fluorescence indicator emits a pale blue fluorescence in short-wave UV light. UV absorbing substances appear as dark-blue to black spots on a light-blue background. |
|