2-MERCAPTOETHANOL for molecular biology, ≥98% (GC/titration
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
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Specifications
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| Synonym | β-Mercaptoethanol |
| 2-Hydroxyethylmercaptan |
| BME |
| Thioethylene glycol |
| Molecular Formula | HSCH2CH2OH |
| Molecular Weight | 78.13 |
| grade | for molecular biology |
| vapor density | 2.69 (vs air) |
| vapor pressure | 1 mmHg ( 20 °C) |
| assay ≥98% | (GC/titration) |
| expl. lim. | 18 % |
| concentration | 14.3 M (pure liquid) |
| refractive index | n20/D 1.500(lit.) |
| bp | 157 °C(lit.) |
| density | 1.114 g/mL at 25 °C(lit.) |
| foreign activity DNase-RNase-protease-none detected | 160 |
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